Looking for the right mate—What we really know on the courtship and mating of Lucilia sericata (Meigen)?

Lucilia sericata is well known for causing myiasis in humans, livestock, pets and wildlife. It also vectors microbial pathogens, including paratuberculosis agents. This species can be exploited in maggot therapy to treat necrotic wounds, particularly those infected by multidrug-resistant pathogens. Despite its high medical and veterinary importance, our knowledge about its courtship and mating behavior is still limited. In this study, we quantified the courtship and mating behavior of L. sericata, shedding light on the potential impact of lateralization of selected behavioral traits during sexual interactions. When a male identified a female, he approached her with head pushing followed by foreleg tapping acts. Courtship lasted 7.65 ± 0.4 s. During copulation attempts, the male continued foreleg tapping acts on the female body, and tried to achieve genital contact. Copula lasted 14.88 ± 0.41 min, while male mating success was 85%. Several courtship and mating traits were found lateralized at population-level. Most of males approached the female with head pushing acts on her left side of the body. Both during courtship and copulation attempt phases, males mainly used the right foreleg to perform leg tapping acts on females. However, the impact of lateralized head pushing and foreleg tapping on the main behavioral parameters characterizing L. sericata courtship and mating was not significant, except for a higher number of foreleg tapping acts during copulation attempts displayed by right-biased males over left-biased ones. Overall, these results contribute to improve our basic knowledge on the reproductive behavior of L. sericata. Besides, selected behavioral parameters characterized here can be exploited as benchmarks to monitor mate quality during mass-rearing, as well as to select males with boosted mating competitiveness, helping to improve the success of SIT programs and behavior-based control tools.